高晓威，博士，副教授，硕士研究生导师，微生物与生化药学教研室主任。主要从事蛋白质工程、酶定向进化、益生菌工程等方面的研究。具体研究内容包括：（1）具有重要工业价值的蛋白质及酶资源的发掘应用；（2）基于非天然氨基酸的蛋白质及酶分子改造；（3）基于益生菌工程的活体药物在疾病诊疗中的应用。

联系方式：

邮箱：gxwwhu@126.com

通讯地址：西南医科大学城北校区（泸州市香林路1段1号）弘润楼426室 646000

学习及工作经历：

2021-至今 西南医科大学 药学院       副教授

2018-2020 西南医科大学 药学院       讲师

2013-2017 武汉大学     生命科学学院 博士

2008-2012 河南师范大学 生命科学学院 本科

主讲课程：

《生物技术制药》、《微生物药物学》、《高等分子生物学》、《人类与微生物》

主持科研项目：

1、四川省科技计划面上项目（NO.2024NSFSC2097），2024-2026年，20万元。

2、中国博士后科学基金面上项目（NO.2020M683364），2021-2022年，8万元。

3、四川省科技计划青年项目（NO. 2020YJ0129），2020-2022年，10万元。

4、神经与肿瘤药物研发全国重点实验室开放基金项目（NO. SKLSIM-2024189），2025-2026年，5万元。

5、泸州市-西南医大科技战略合作项目（NO. 2024LZXNYDJ054），2024-2026年，10万元。

6、泸州市-西南医大科技战略合作项目（NO. 20YKDYYJC0030），2020-2023年，10万元。

7、西南医科大学青年科技人才培育计划，2022-2024年，10万元

8、西南医科大学应用基础研究项目（NO. 2021ZKMS045），2021-2024年，10万元

9、叙永县人民医院-西南医科大学合作项目（NO. 0500160098），2024-2025年，5万元

10、西南医科大学青年基金项目（NO. 2020ZRQNA007），2020-2022年，2.0万元

发表论文：

以第一作者或通讯作者累计发表SCI科研论文20余篇，代表性论文:

1、Yanhong Yang, Jing Zhang, Jian Yang, Huiwen Luo, Yingjie Sun, Famin Ke, Qin Wang, Xiaowei Gao*. Directed evolution of the fluorescent protein CGP with in situ biosynthesized noncanonical amino acids. Applied and Environmental Microbiology, 2024, 90(4):e0186323.

2、Kamin Ke, Yingjie Sun, Tingting He, Wenbo Liu, Yijiao Wen, Siyuan Liu, Qin Wang, Xiaowei Gao*. Effects of feather hydrolysates generated by probiotic Bacillus licheniformis WHU on gut microbiota of broiler and common carp. Journal of Microbiology, 2024, 62(6):473-487.

3、Haixia Peng, Manyu Liang, Jing Zhang, Wenbo Liu, Yanhong Yang, Yingjie Sun, Famin Ke, Yijiao Wen, Siyuan Liu, Bilin Xu, Xiaowei Gao*. Identification and characterization of a versatile keratinase, KerZJ, from Stenotrophomonas sp. LMY. World Journal of Microbiology and Biotechnology, 2024, 40:30.

4、Manyu Liang, Yanhong Yang, Jing Zhang, Yingjie Sun, Haixia Peng, Huawei Yi, Qin Wang, Xiaowei Gao*. Use of noncanonical amino acids in genetic code expansion-based therapeutics: Effects on mouse gut microbiota. Microbial Biotechnology, 2023,16(12):2278-2291.

5、Jing Zhang, Manyu Liang, Lijuan Wu, Yanhong Yang, Yingjie Sun, Qin Wang, Xiaowei Gao*. Bioconversion of feather waste into bioactive nutrients in water by Bacillus licheniformis WHU. Applied Microbiology and Biotechnology, 2023, 107:7055-7070.

6、Lijuan Wu, Xiaoqing Lao, Yazeng Wu, Jing Zhang, Manyu Liang, Yanhong Yang, Xiaowei Gao*. Insights into effects of sodium phytate on gut microbiome of mice by high-throughput sequencing. Biotechnology & Biotechnological Equipment, 2023, 37:2220825.

7、Manyu Liang, Jing Zhang, Yanhong Yang, Yi Xia, Lintao Liu, Li Liu, Qin Wang, Xiaowei Gao*. Nattokinase enhances the preventive efects of Escherichia coli Nissle 1917 on dextran sulfate sodium induced colitis in mice. World Journal of Microbiology and Biotechnology, 2022, 39:8.

8、Famin Fe, Peijuan Xie, Yanrong Yang, Liu Yan, Ailing Guo, Jian Yang, Jing Zhang, Li Liu, Qin Wang, Xiaowei Gao*. Effects of Nisin, Cecropin, and Penthorum chinense Pursh on the Intestinal Microbiome of Common Carp (Cyprinus carpio). Frontiers in Nutrition, 2021, 8:729437.

9、Qin Wang, Xinmeng Jiang,Yanping Gao, Liping Yin, Xiaoyao Wei, Kai Guo, Xiaowei Gao*, Li Wang*, Chun Zhang*. Studies on Biosynthesis of Chiral Sulfoxides and Omeprazole by Using P450 119 Peroxygenase and Its Mutants. ChemistrySelect, 2022, 7(47):e202204031.

10、高晓威，韦思平，王钦. 遗传密码子扩展技术在蛋白质及多肽类药物中的应用. 生物化学与生物物理进展, 2022, 49 (1):183-201.

11、Xianchao Pan, Jian Yang, Peijuan Xie, Jing Zhang, Famin Ke, Xiurong Guo, Manyu Liang, Li Liu, Qin Wang, Xiaowei Gao*. Enhancement of activity and thermostability of keratinase from Pseudomonas aeruginosa CCTCC AB2013184 by directed evolution with noncanonical amino acids. Frontiers in Bioengineering and Biotechnology, 2021, 9:770907

12、Huawei Yi, Jing Zhang, Famin Ke, Xiurong Guo, Jian Yang, Peijuan Xie, Li Liu, Qin Wang, Xiaowei Gao*. Comparative analyses of the transcriptome and proteome of Escherichia coli C321.∆A and further improving its noncanonical amino acids containing protein expression ability by integration of T7 RNA polymerase. Frontiers in Microbiology, 2021,12:744284

13、Zhijie Zhang, Jian Yang, Peijuan Xie, Yanping Gao, Jun Bai, Chun Zhang, Li Liu, Qin Wang, Xiaowei Gao*. Characterization of a thermostable phytase from Bacillus licheniformis WHU and further stabilization of the enzyme through disulfide bond engineering. Enzyme and Microbial Technology, 2020, 142:109679.

14、Dong Yi, Juan Xing, Yanping Gao, Xianchao Pan, Peijuan Xie, Jian Yang, Qin Wang, Xiaowei Gao*. Enhancement of keratin-degradation ability of the keratinase KerBL from Bacillus licheniformis WHU by proximity-triggered chemical crosslinking. International Journal of Biological Macromolecules, 2020, 163:1458-1470.

15、Famin Ke, Yanping Gao, Li Liu, Chun Zhang, Qin Wang, Xiaowei Gao*. Comparative analysis of the gut microbiota of grass carp fed with chicken faeces. Environmental Science and Pollution Research, 2020, 27: 32888-32898.

16、Xiaoyao Wei^#, Chun Zhang^#, Xiaowei Gao^#, Yanping Gao, Ya Yang, Kai Guo, Lin Pu, Qin Wang*. Enhanced Activity and Substrate Specificity by Site-Directed Mutagenesis for the P450 119 Peroxygenase Catalyzed Sulfoxidation of Thioanisole. ChemistryOpen, 2019, 8(8):1076-1083.

17、高晓威，唐晓峰，唐兵. 超嗜热古菌的ATP非依赖型蛋白酶和肽酶研究进展. 微生物学报，2017, 57(9): 1373-1382

18、Xiaowei Gao, Jing Zeng, Huawei Yi, Fang Zhang, Bing Tang, Xiao-Feng Tang. Four Inserts within the Catalytic Domain Confer Extra Stability and Activity to Hyperthermostable Pyrolysin from Pyrococcus furiosus. Applied and Environmental Microbiology, 2017, AEM. 03228-16.